Evaluation of the Cytotoxic Activity of Crude Aqueous Extracts of Some Medicinal Plants of Kashmir

The objective of this study was to evaluate the cytotoxicity potential of aqueous extracts of Nepeta cataria, Fumaria indica, Borago officinalis, Adiantum capillus, Levandula stoeches on HeLa cell line. Leaves of plants were washed with distilled water, dried in shade, grinded to fine powder and stored in airtight container at room temperature in the dark until used. The powdered samples were subjected to extraction using distilled water. Different concentrations of the extracts of herbals were prepared. Cytotoxicity study was carried out by The MTT cell proliferation assay on HeLa cell line which measures the cell proliferation rate and conversely, when metabolic events lead to apoptosis or necrosis, there is reduction in cell viability. Fumaria indica exhibited cell viability ranging from 89.60 to 100 per cent against the extract concentration of 5 to 100 μg/ ml with IC50 (μg/ml) on HeLA cell line as -581.79 Adiantum capillus extract revealed cell viability from 81.91 to 100 per cent against the extract concentration of 5 to 100 μg/ml with IC50 (μg/ml) on HeLA cell line as 36.96. Nepata cataria extract showed cell viability ranging from 84.36 to 96.09 per cent against the extract concentration of 5 to 100 μg/ml with IC50 (μg/ml) on HeLA cell line as -112.07. In case of Levandula stoeches extract per cent cell toxicity ranged from 0 to 41.77 against the extract concentration of 5 to100 μg/ml. Borago officinalis extract exhibited per cent cell toxicity ranging from 4.27 to 43.17 against the extract concentration of 5 to100 μg/ml with IC50 (μg/ml) on HeLA cell line as 26.91.